Composite

Part:BBa_K731255:Design

Designed by: Daniele Rossetto   Group: iGEM12_UNITN-Trento   (2012-08-03)


Inducible araC-pBAD promoter with GFPmut3b, weak RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1876
    Illegal SapI site found at 961


Design Notes

Source

araC-pBAD from E. coli genome, GFPmut3b is from the registry (part BBa_E0840)

References